![]() ![]() Try to lower the mismatch value in such case. However, specifying a larger mismatch value may make it more difficult to find such specific primers. The larger the mismatches (especially those toward 3' end) are between primers and the unintended targets, the more specific the primer pair is to your template (i.e., it will be more difficult to anneal to unintended targets). This requires at least one primer (for a given primer pair) to have the specified number of mismatches to unintended targets. Note that the organism field is ignored for custom database. A maximum of 20 assembly accessions are allowed. You can use your own sequences including nucleotide accessions, genome assembly accessions (such as GCF_000001635.27) or FASTA sequences as a search database. This database is recommended if you are not considering variations represented by alternate loci. Mitochondrion and plastid genomes are also included where applicable.Īlthough sequences in this database are completely covered by the Refseq representative genomes database, it does not contain the alternate loci and thus avoids sequence redundancy introduced by including alternate loci. These are Refseq representative genomes from primary chromosome assemblies (i.e., no alternate loci) for many eukaryotic organisms. Genomes for selected eukaryotic organisms (primary assemblies only): This contains all RNA entries from NCBI's Reference Sequence collection Mitochondrion genomes are included where applicable. For other species, genomes from diverse isolates of the same species may be included. ![]() For the eukaryotes, only one genome is included per species (However, alternate loci of eukaryotic genomes are included where applicable). This database contains minimum redundancy in genome representation. These genomes are among the best quality genomes available at NCBI. This database contains NCBI RefSeq Reference and Representative genomes across broad taxonomy groups including eukaryotes, bacteria, archaea, viruses and viroids. MindMup also integrates with Office365 and Google authentication for easy account management.This contains mRNA only from NCBI's Reference Sequence collection Administer easilyĪdministrators can easily control sharing settings for the entire organisation. Quickly add images and icons to your maps to provide context and illustrations. MindMup for Google Drive also allows you to link to other project documents on Google Drive easily. Attach documentsĪdd longer text, videos and spreadsheets to your maps. Maps saved to MindMup Cloud support concurrent editing for teams and classrooms, enabling you to instantly see updates made to your map by other users. Manage project progress easily with icons and priorities. MindMup Atlas automatically adds images and contextual information to ensure that your maps show up nicely on Twitter, Facebook, Google+, LinkedIn and Pinterest.Īdd measurements to map nodes to automatically calculate cost, completion and other important factors. Use a frictionless planning canvas to capture and organise your thoughts, and then put them into a powerful storyboard to turn a hierarchy of ideas into a sequence of slides. Use notes and attachments to quickly create a structure for an article or a writing assignment, then create a document outline and continue editing in your favourite writing tool Create storyboards ![]()
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